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Figure 4


Fig. 4. Snail transcription factors mediate the effects of Wg signaling in peripheral eye development. With the exception of C, all micrographs are flat mounted pupal retinas of ~40 hours APF. (A-A')A dsh[V26] clone at the eye margin marked black by absence of GFP showing ectopic cone cells stained with Cut (red, arrowhead). (B)A Df(3L)H99 clone in the eye margin marked black by the absence of GFP showing ectopic cone cells (red). The cone cells from more interior ommatidia are not visible at this focal plane. (C) Whole-mount views of adult eyes uniformly ectopically expressing Wg and Escargot under the GMR promoter. High level Wg and Escargot result in small heavily pigmented eyes. (D-D") A dsh[V26] clone at the eye margin marked black by absence of GFP and showing retinal protrusion caused by the inappropriate survival of the peripheral ommatidia stained for Elav (blue). The PR, highlighted by coracle staining (red) is significantly reduced in the clone (arrowhead) compared with the adjacent wild-type patch (arrow). (E) A dsh[V26] clone at the eye margin marked black by absence of GFP showing ectopic primary pigment cells stained with BarH1 (red). (F) A clone doubly mutant for esg and sna, and overexpressing two different worniu RNAi constructs (triple mutant clone) in the eye margin marked by GFP (green). (F',F") Ectopic photoreceptor cells stained for Elav (arrowheads, red) are present in the clone. The photoreceptors of the more internal ommatidia are not visible at this focal plane. (G) A triple mutant clone marked by GFP (green) at the eye margin. (G',G") Ectopic cone cells (red) are present in the clone (arrowheads). Cone cells of interior ommatidia are not fully visible at this focal plane. (H,H') A triple mutant clone in the eye margin marked by GFP (green) showing a significant reduction of the pigment rim (red, arrowheads). (I,I') A higher magnification view within a triple mutant clone (clone marker not shown) showing no effect on homothorax expression (green) in both ectopic (arrowheads) and standard DRO ommatidia.