Fig. 9. Melanocyte regeneration deficit in kit^j1e99 larvae. (A) Larval melanocytes were counted in the untreated (blue line) and MoTP-treated (14-72 hpf; red line) kit^j1e99 larvae at the permissive temperature (25°C) for the temperature-sensitive allele. The first few melanocytes appear approximately 3 days post-MoTP treatment in the MoTP-treated kit^j1e99 animals at 25°C. The number of melanocytes in MoTP-treated larvae reaches approximately 15 by 9 dpf (6 days after removal of MoTP, red line in A), whereas age-matched untreated kit^j1e99 animals have approximately 350-400 melanocytes (blue line in A). (B,C) Note that the regenerated melanocytes exhibit a stereotyped spacing morphology in the dorsum (white arrowheads in C). B and C are untreated and MoTP-treated kit^j1e99 larvae at 7 dpf, respectively. Scale bar: 500 µm