Fig. 2. Abnormal morphogenesis and tissue-specific gene expression in dic-1 (RNAi) embryos. RNA interference was carried out from the L4 stage by feeding bacteria producing double-stranded RNA. (A) Time lapse microscopy of embryonic development using Nomarski optics. Developmental stage and time post fertilization (in minutes) are indicated for each wild-type embryo. dic-1(RNAi) embryos laid at the same time as the wild-type embryos were arrested after the bean cell stage but before the comma stage. (B) Embryos obtained after RNAi of dic-1 in wild-type N2 and ced-3(n717), after longer incubation than in A. Cavities are marked with arrowheads. (C) Wild-type embryos at the threefold stage and dic-1(RNAi) embryos at the same time post fertilization, showing P^snap-25::gfp expression in neurons, ajm-1::gfp expression in the junctions of intestinal cells and unc-15::gfp expression in muscle cells. Scale bars: 10 µm.