Fig. 5. Immunolocalization of DIC-1 in mitochondria. (A-C) Immunolocalization of DIC-1 in embryos and germline by fluorescence microscopy. (A) Wild-type and dic-1(RNAi) embryos. (B) In wild-type gonads, DIC-1 was restricted to the cytoplasm from the distal tip cells to the oocytes. (C) Co-localization of DIC-1 with cytochrome c oxidase subunit 1 within mitotic cells of the gonad. (D) Electron micrograph (left) showing immunogold particles (arrowheads) in mitochondria: the bracketed area is enlarged in the right micrograph. Scale bars: 10 µm in A; 50 µm in B; 25 µm in C; 0.2 µm in D.