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Figure 5


Fig. 5. Expression of myocardial and endocardial markers in Gata4T2del mutant hearts. (A,B) In situ hybridization for Nppa (A) and Tbx2 (B) in E9.5 embryos, showing intact expression in Gata4T2del hearts. Asterisk indicates the EC. (C) In situ hybridization for Notch1 in E10.5 embryos, showing unperturbed expression in AV endocardium (arrowheads). (D) Alcian Blue staining of acidic glycosaminoglycans, including hyaluronic acid, in E10.5 control and mutant cushions. (E,F) Cdh5 immunostaining of E9.5 embryo sections. Note the bunched up phenotype of the mutant AV endocardium compared with control AV endocardium (arrowheads). Panels in F are higher magnifications of the sections shown in E.