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Figure 1


Fig. 1. Gli2-lacZ expression in embryonic mammary buds. (A-C) Whole-mount X-gal staining of Gli2-lacZ embryos. (A) At E11, expression is seen in an arc between the fore- and hindlimbs (arrow) and in the somites (som, arrowhead). (A, inset) A cross-section through this embryo shows staining is found within the dermal mesenchyme (mes) but not in the epithelial layer (epi, arrow). (B,C) At E14.5 strong staining is seen in both hair placodes and mammary buds (1,3,4, arrows). (D-G) Cross-sections through Gli2-lacZ embryos stained with X-gal. Staining is seen within the basal epithelial layer (epi) and surrounding stroma (str) of E14.5 mammary buds (D) and E16.5 mammary sprout (E). Epithelial Gli2-lacZ expression is restricted to a basal subset of p63-(F) and K14-(G) positive cells.