Fig. 3. AHNPs maintain viability and assume glial phenotypes upon ventricular transplantation. (A) AHNPs cells injected into the right lateral ventricle of postnatal day 3 mice were detectable with HNA in periventricular tissue adjacent to injection site (^*). (B) HNA⁺ cells (boxed in a) were primarily located within 100 µm of the ventricular wall in the ependymal and subependymal cell layer. (C-E) HNA⁺ cells (red) integrating into the LV wall display conserved morphology of astrotypic cells, and frequently co-express GFAP (green). (F) Confocal micrograph shows GFAP⁺ process extension from an AHNP into the subependymal zone. (G,H) Activated dendrimer transfection of Pax6-eGFP of 30 PDs AHNPs 3 days post-transfection. (I) Lentiviral-eGFP transfection of 30 PD AHNPs (20 moi). Scale bars: 40 µm in B; 25 µm in C-E; 8 µm in F; 20 µm in G-I. Images in C-F,H are counterstained with DAPI.