Fig. 3. Hex amplifies the transcriptional activity of ß-catenin in HEK 293T cells. (A) Reporter plasmids used in the transfections: TOPflash, a reporter gene containing reiterated Tcf-binding sites upstream of the tk promoter and FOPflash, a mutated version of TOPflash used as a negative control. (B,C) Wnt reporter gene activity in response to transfection of Hex and Wnt pathway components. The indicated DNA was co-transfected with either TOPflash or FOPflash. Hex, Hex-VP2 and ß-catenin were used at 100 ng. Stabilised ß-catenin (stab. ß-catenin), LefdNß-CTA and Xtcf3-VP2 at 90 ng. Values are represented as fold induction of the indicated reporters. Experiments were carried out in triplicate.