Fig. 5. Validation of Hex targets in Xenopus embryos. (A) Expression of candidate Hex target genes in response to depleting endogenous Hex. In situ hybridisation for Xtle4, Xnr1, Xnr2, Xnr5 and Xnr6 in Hex MO depleted and rescued embryos. Embryos were injected as in Fig. 1F with the indicated MO/RNA combination and analysed by in situ hybridisation at stage 10.5. Where CHX is indicated, embryos were transferred to media containing CHX before MBT and collected 90 minutes later for fixation. As CHX slightly delays development, some of these embryos do not present evident dorsal lips. (B) Expression of candidate Hex target genes in response to Hex-VP2. In situ hybridisation of embryos injected with Hex-VP2 and assayed for the expression of the same set of candidate genes as in A. Embryos were injected at the four-cell stage on the dorsal side alongside nucGFP RNA. Embryos were bisected for better observation of the internal expression. Dorsal is towards the right.