Fig. 6. Xtle4 overexpression antagonises Hex-amplification of Wnt signalling. (A)In situ hybridisation for the expression of Xnr3 and Cerberus at stage 10.5. Embryos were injected at the two-cell stage in both blastomeres with 500 pg Hex, ß-catenin and/or 3000 pg Xtle4. (B) Activity of Hex and ß-catenin from the TOPflash Wnt reporter gene in the presence of Xtle4. The indicated DNA was co-transfected with either TOPflash or FOPflash in HEK 293T cells. Hex, Hex-VP2 and ß-catenin were used at 100 ng, Xtle4 at 90 ng. Values are represented as fold induction of the indicated reporters. Experiments were carried out in triplicate.