Fig. 7. PAR-6 puncta that do not contain PAR-3 are rare in cdc-42(RNAi) embryos. (A-F) Immunofluorescence images of anterior cortical patches of control embryos (A-C) or cdc-42(RNAi) embryos (D-F), showing the distributions of PAR-3 (red) and GFP::PAR-6 (green) puncta. In A, examples of puncta positive for only anti-PAR-3 are marked with red asterisks. In B, examples of puncta positive for only anti-GFP are marked with green asterisks. Note the larger puncta and the much more extensive overlap of anti-PAR-3 and anti-GFP positive puncta in D-F. Embryos examined were at late prophase of the first mitotic cell cycle.