Fig. 6. Sox8 and Sox9 regulate Sox10 expression in animal explants. (A) Real-time RT-PCR of animal explants isolated from embryos injected with Sox8GR, Sox9GR or Sox10GR. The histograms indicate the relative expression levels of Sox8 (yellow), Sox9 (blue) and Sox10 (green) in animal explants collected 4 hours after dexamethasone treatment (+Dex). (B) In animal explants, Sox8GR, Sox9GR and Sox10GR strongly induce Sox10 expression after 4 hours of dexamethasone treatment (+Dex). The induction of Sox10 by Sox8GR, Sox9GR or Sox10GR occurs independently of protein synthesis (+CHX), indicating that not only can Sox10 regulate its own expression but that Sox8 and Sox9 can directly activate Sox10. (C) Hierarchy of SoxE genes activation in the developing neural crest.