Fig. 3. PI-PLC and EphA3-Fc could alleviate the inhibitory activity of the caudal hindbrain. (A,B) The experimental procedures followed illustrated using the same grafting scheme as in Fig. 2B. With normal culture medium (A1) or with only hFc added to culture medium (B1), almost all cC-VC axons were prevented from entering the caudal graft. However, adding PI-PLC (A2) or unclustered EphA3-Fc (B2) to the culture medium led to many axons entering the graft, reducing the inhibition in the graft. White dots outline the grafts. Scale bar: 400 µm.