Fig. 5. EphA5 and EphA3 are expressed in presumptive cC-VC neurons, and ephrin A2 protein is distributed in a gradient in caudal hindbrain. (A,B) EphA5 and EphA3 in situ hybridisation on stage 25 hindbrains, respectively. The blue brackets in both images indicate expression domains correlating with the location of cC-VC neurons. (C) Ephrin A2 immunohistochemistry on a lateral parasagittal section of a stage 26 brainstem. The graded distribution of ephrin A2 in the caudal hindbrain is indicated by arrows a, b and c. Inset shows ephrin A2 protein in tectum and cerebellum from the same brain. (D) Ephrin A2 immunostaining on three transverse sections from a stage 26 hindbrain. a-c correspond approximately to positions a-c in C. CB, cerebellum; gV, trigeminal ganglion; ov, otic vesicle. Scale bar: 400 µm in A,B; 200 µm in C, inset in C and D.