Fig. 1. PKD-2 localizes to cilia and ER in male-specific sensory neurons. (A-G) Images are projected z-series obtained by laser-scanning confocal microscopy unless indicated otherwise. (A) A lateral view of an adult male expressing Ppkd-2::GFP (pkd-2 promoter driving GFP expression). GFP is expressed in four CEMs in the head, 16 ray B neurons, and the HOB neuron in the tail. Scale bar: 100 µm. (B,C) PKD-2::GFP localizes in the cell bodies (arrows), small dendritic and axonal puncta, and ciliary endings (arrowheads). Scale bars: 10 µm. (B) CEMs, (C) RnB ray (three arrows) and HOB (one arrow) neuronal cell bodies, and ciliary regions (ciliary membrane and base, arrowheads). (D,E) GFP-tagged TRAM expressed under (Ppkd-2::TRAM::GFP) localizes in cell bodies (arrows), dendritic puncta and ciliary bases (open arrowheads), but not the cilium proper. Scale bars: 10 µm. (D) CEMs, (E) HOB and RnB neurons. (F,G) GFP-KDEL ER marker driven by the pkd-2 promoter shows reticular structure in cell bodies and is excluded from ciliary and dendritic compartment of the cells. Open arrowheads indicate the absence of GFP in cilia. (H) Epifluorescence images showing overlapping expression of PKD-2::GFP and Ppkd-2::TRAM::DsRed2 in RnB cell bodies.