Fig. 2. PKD-2::GFP moves rapidly and bidirectionally in dendrites. (A) A montage of PKD-2::GFP particles shows both anterograde (black arrows, from the cell body to cilium) and retrograde (white arrowheads) movement. The broken white line indicates stationary particles. Scale bar: 5 µm. (B) A kymograph depicting PKD-2::GFP particle motility in the dendrite. The horizontal and vertical axes represent time and distance, respectively. Vertical scale bar: 5 µm. The left arrowhead illustrates the changes from anterograde to retrograde and the right arrowhead reflects retrograde to anterograde changes. Saltatory movement is also detected (arrows). (C) Distribution of PKD-2 particle velocities in dendrites. The particles have distinct velocities for retrograde (light boxes) and anterograde (dark boxes) directions. The velocities are shown as the average ± s.e.m. Time lapse movies are available upon request.