Fig. 5. Most spindles align with the long axis at prophase. The deviation of the spindle axis from the long axis was measured in parallel (symmetrically) dividing fixed cells at different time-points during mitosis (A-C). Charts on the left show the distribution of the raw data; y-axis=0° corresponds to long axis; each triangle represents one measurement. The radial line indicates the median angle deviation. Graphs on the right show the percentage of cells in angle classes of 20°. The majority of cells have their spindles aligned within 20° of the long axis throughout the cell cycle. (D) Cells with a spindle axis perpendicular to the surface were measured at prophase (n=41 cells).