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Figure 6


Fig. 6. Astral microtubules are important for spindle alignment into the long axis. The deviation of the spindle axis from the long axis was measured in cells treated with nocodazole. (A,D) Antibody staining for {alpha}-tubulin in treated (A) and control (D) cells. (B,C) A reduction of astral microtubules leads to an increase of the median deviation from the long axis compared with the controls (E,F). Eighty-five cells were analysed in ten nocodazole-treated embryos and 75 cells in 12 control embryos. The difference between the median angles is statistically significant by the Mann-Whitney test (P<0.001).