Fig. 4. Analysis of junctional marker expression during neurulation.
(A-P) Cross sections through the anterior neuroepithelium of wild-type
(A,C,E,G,I,K,M,O), N-cad MO-injected (B,P) and
N-cadp79emcf mutant (D,F,H,J,L,N) embryos. Developmental
stages are indicated in the upper right corner; dorsal is towards the top in
all panels. Embryos were labeled with 
-ß-cat (A-J), -ZO-1
(K,L), -aPKC (M,N), DAPI (A-N) and Alexa-488-phalloidin (O,P). Insets
in A-D show higher magnifications of cells. Asterisk in I indicates the
position of the forming ventricle, arrowheads point to midline apical
labeling, arrows in N point to apical -aPKC labeling, arrows in P show
rosettes, and the dotted line in P indicates apical localization of
phalloidin. Scale bar: 20 µm.