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Figure 5


Fig. 5. MES-4 binding and methylation of H3K36 do not appear to depend on transcription elongation. Comparison of MES-4 and H3K36me2 distribution and levels in ~24-cell wild-type and ama-1(RNAi) embryos. Anterior is to the left. DNA is stained red with anti-acetylated H4 (A,D) or PA3 (B,C,E,F); staining of CTD Ser2 phosphorylation (pSer2), MES-4 and H3K36me2 is green. (A-C) Wild-type embryos. (D) ama-1(RNAi) embryo showing effective depletion of pSer2. (E,F) ama-1(RNAi) embryos showing apparently normal levels and distributions of MES-4 and H3K36me2. Scale bar: 10 µm.