Fig. 7. Microarray analysis reveals derepression of X-linked genes in mes-4 mutant germ lines. Microarray analysis was performed using linearly amplified RNA from gonads dissected from wild-type and mes-4(bn85) M⁺Z^- adult hermaphrodites. (A) Green and red tick marks show the chromosomal positions of the 71 genes that are up- or downregulated at least 1.8-fold in mes-4 mutants relative to wild type (P<0.05, two-tailed paired t-test). Green, 67 genes that are upregulated in mes-4 mutant gonads; red, four genes that are downregulated in mes-4 mutant gonads. Gene IDs and fold differences are shown in Table S2 in the supplementary material. (B) Histograms showing the number of genes on each chromosome that displayed various mean hybridization intensities. The total number of genes sampled on each chromosome is in parentheses in the key. (C) Histograms showing the percentage of X-linked genes that displayed various mean hybridization intensities. Pink, 61 X-linked genes that are upregulated >1.8-fold in mes-4 mutant gonads; blue, all 2423 analyzed genes on the X chromosome. The units on the x-axis change at 10,000.