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Figure 5


Fig. 5. Man1{Delta}/{Delta} embryos exhibit defective vascular remodeling. (A-H) Whole-mount immunostaining with an antibody to PECAM1. (A,B) The yolk sac and (C,D) embryo proper at E10.0. Closer views of blood vessels in the head region (E,F) and intersomitic vessels (G,H) of the embryos shown in C and D, respectively. (I,J) Immunohistochemical staining for smooth muscle {alpha}-actin in transverse sections of E10.0 embryos of wild-type and mutant embryos. (K-P) Electron microscopic analysis of the perivascular walls. (M,N) Higher magnification images of the insets in K and L, respectively. White arrowhead indicates a mural cell; black arrowheads indicate endothelial cells; arrows indicate tight junctions. (O,P) Higher-magnification views of the tight junction between endothelial cells in M and N. da, dorsal aorta. Scale bars: 200 µm in A-H; 20 µm in I,J; 10 µm in K-N; 1.0 µm in O,P.