Fig. 2. Cyclin D2 (brown) is not expressed at high levels in the EGL until later in cerebellar development. (A-H) The E16.5 (A, with high-power views of boxed areas shown in B-D) and P0 (E, with high-power views of boxed areas shown in F-H) EGL show little nuclear cyclin D2 staining. Enlarged views of the ventricular region (B,F), anterior EGL (C,G) and posterior EGL (D,H) are shown. Only a few nuclei in the posterior EGL (arrow in D) are weakly positive, in comparison with strong staining of the ventricular zone of the midbrain and of cells deeper within the cerebellum (arrowheads in C,G). (I,J) Strong cyclin D2 expression is seen in the outer half to two-thirds of the EGL at P6 (I, with high-power of view of boxed area in J). (K,L) Scattered EGL cells are positive (arrows) at P12 (K, with high-power of view of boxed area in L). Brackets indicate the width of the EGL. Nuclei are lightly stained purple with Hematoxylin and Eosin. Scale bars: 50 µm.