Fig. 4. Functional complementation of mrp-4(cd8) and localization of MRP-4::GFP. (A) Confocal micrographs of `1.5-fold' stage embryo or adult hermaphrodites expressing a transcriptional fusion of the mrp-4 promoter to GFP. (B) Percent of embryos that hatch and percent of hatched embryos that develop to adults in the indicated strains. (C) Confocal micrographs of `1.5-fold' stage embryos laid by hermaphrodites carrying the MRP-4::GFP transgene (green in merge) and grown on plates containing LysoTracker Red (red in merge). All images of the same marker were taken with the same exposure and at the same magnification. (D) Quantitation of the surface area of the LysoTracker Red granules in intestinal cells shown in C. Wild-type and cup-5(zu223) values are included for comparison. One pixel is 0.01 µm². (E) Confocal micrographs of `1.5 fold' stage embryos laid by hermaphrodites carrying the MRP-4::GFP transgene (green in merge) and stained with Nile Red (red in merge). All images of the same marker were taken with the same exposure and at the same magnification. (F) Quantitation of the intensity per surface area of the Nile Red granules in intestinal cells shown in E. Wild type and cup-5(zu223) values are included for comparison. The alleles in all panels are mrp-4(cd8) and cup-5(zu223). Scale bar: 10 µm in C,D. Large arrows indicate staining of intestinal cells. The MRP-4::GFP transgene also carries a marker that expresses GFP in pharyngeal cells (small arrows).