Fig. 3. Ventral body wall myoblasts appear to migrate as mesenchymal cells.
(A) lbx1-expressing myoblasts of a stage 37/38 tadpole emerge
at the somite cleft (arrows, most posterior somite with emerging myoblasts
shown). (B) Another hypaxial myoblast-specific marker, tbx3,
can be seen expressed in cells migrating out of the somite clefts in a stage
31 tadpole (arrows). Myotomes in A and B are labeled with 12/101 (brown).
(C,D) lbx1 expression is found in long-range type
hypaxial myoblasts. lbx1 expression in the geniohyoideus can be seen
in a ventral view of a stage 42 tadpole (C, arrowheads), which co-localizes
with 12/101 (D). (E,F) lbx1 is also found in the
migrating muscles of the hindlimb of a stage 53 tadpole (E, arrows), similar
to pax3 expression at the same stage (F, arrows) (limbs are shown
from a lateral view, distal end towards the left).