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Fig. 1. GDF3 is specifically expressed in the undifferentiated state of embryonic stem cells. (Left panel) RT-PCR of GDF3 expression levels in H1 human embryonic stem cells (HES) in the continued presence (CM) and absence (no CM) of conditioned media for 7 days. (Right panel) GDF3 RT-PCR in 129Ola mouse ES cells in the presence or absence of LIF for 3 days. OCT4, SOX2 and NANOG are markers of pluripotency. ß-actin is shown as a loading control; no RT control showed no signal.