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Fig. 3. Immunoprecipitation experiments. (A) Immunoprecipitation of GDF3 and BMP4 from animal caps of frog embryo. First and second panels show 10% crude levels input for immunoprecipitation. Third panel shows immunoprecipitation with FLAG-GDF3, blotted for HA-BMP4; fourth panel shows immunoprecipitation with HA-BMP4, blotted for GDF3. Prepro forms are indicated (**, <<) and mature forms are indicated (*, <). (B) Immunoprecipitation by BMP4-HA or activin-HA using m{alpha}HA antibody and blotting with goat {alpha}GDF3. The GDF3 prepro (**) form is shown in the upper panel and mature form (*) in the lower panel. (C) Immunoprecipitation of GDF3 by BMP4-HA from CM of COS cells. GDF3 and BMP4 were transfected into separate COS cells (lanes 1,2,4,5) or together in the same COS cells (lanes 3,6). In lane 5, COS CM from separately transfected GDF3 and BMP4 cells was mixed together for the immunoprecipitation. Only the prepro form of GDF3 is shown.