Fig. 4. Protein analysis. Western blot of BGN1 (A) and Jasmine (B) human ES cells transfected with empty vector (V) or Gdf3 (G). Cells were either maintained undifferentiated in CM or cultured in the absence of CM (non CM), with and without rhBMP4 treatment for 3 days. OCT4, NANOG and SOX2 are markers of stemness. Cyclophilin B and tubulin are shown as loading controls.