Fig. 6. GDF3 expression during development. (A) RT-PCR of GDF3 throughout early mouse embryo development. HPRT is shown as a loading control and HPRT no RT as a negative control. (B) Immunofluoresence (IF) of GDF3 protein [red (middle)] as detected by goat GDF3 and counterstained with Sytox Green nuclear stain [green (left)], merged in the right panels. The lower panels show a no primary antibody control. Magnification is 10x. (C-F) E7.5 (C), E8.0 (D) and E8.5 (E) embryo in-situ hybridization with GDF3 anti-sense probe. F shows 10-µm sections of whole-mount in-situ hybridizations. In all in-situ experiments, a sense probe showed no specific staining.