Fig. 1. Drosophila Dok sequences and their relationship to the mammalian Dok proteins. (A) Alignment of the Ddok PH domain with mammalian Dok1, Dok2 and Dok3. Identical amino acids are indicated by shading. (B) Alignment of the Ddok PTB domain with the PTB domains of mammalian Dok1-Dok3. (C) Schematic representations of Ddok and Dok1-Dok3, showing C-terminal tyrosines predicted to be phosphorylated by NetPhos. The line joining diamonds in Ddok shows the region of overlap of the Ddok clones obtained in the two-hybrid screen. (D) A phylogenetic tree of Ddok and the mammalian Dok nucleotide sequences compiled using the Clustal W method within the Lasergene Navigator program.