Fig. 3. Ddok gene organization and the phenotypes of Ddok^PG155/Y male `escapers'. (A) Ddok gene organization, showing the position of the P-element insertion in the Ddok^PG155 line. (B) RT-PCR with Ddok and RpS3 (control) primers. Lane 1, 100 bp DNA markers; lane 2, wild-type (WT) flies, with expected 223 bp product; lane 3, Ddok^PG155/Y flies, no detectable product; lane 4, no reverse transcriptase (-RT). (C) The wings of the male escapers are shriveled (b,c) compared with wild-type wings (a). (D) Defects (arrowed) in thorax (b), head (d) and eye (f) of the male escapers compared to wild-type thorax (a), head (c) and eye (e). Male escapers exhibit a split thorax phenotype (b) with loss of bristles at the dorsal midline (arrow). Occasionally, the escapers also show loss of the anterior orbital bristle in the head (d; asterisk indicates the normal developmental process) and irregularly placed bristles (f) in the eyes (arrows).