Fig. 4. Characterization of Ddok^PG155 mutant embryos. (A) Bright-field photographs of cuticle preparations of wild-type and Ddok^PG155(GLC) embryos. Ddok^PG155(GLC) cuticles exhibit a severe dorsal open phenotype with large anterior holes (arrow). (B) Ddok immunofluorescent antibody staining of lateral epidermal cells of stage 13 embryos. Arrow and hatched line indicate LE. (C) Anti-Fasciclin III antibody staining of epidermal cells of stage 12 embryos. (D) In situ hybridization to dpp mRNA in Ddok^PG155 GLC reveals a loss of dpp mRNA specifically from the LE cells (lateral stripes, arrows) in lateral views of stage 11 (a,b), and in dorsal views of stage 13 (c,d), embryos. dpp mRNA staining in other structures of the stage 11 and stage 13 mutant embryos is indistinguishable from that in wild-type embryos.