Fig. 6. Epistatic analysis of Ddok and components of the JNK signaling pathway. Ddok^PG155 GLC flies were crossed to males bearing JNK pathway transgenes or mutations. Embryos bearing heat-shock transgenes were heat shocked for 30 minutes at 37°C at stage 9-11, and then kept at 25°C. Cuticle preparations of (A) wild-type embryos and Ddok^PG155 mutant (Ddok^*) embryos, and (B-F) Ddok^PG155 mutant embryos (right), (B) expressing a constitutively activated form of Jun (hs-SEjun^asp), (C) heterozygous for puc^E69, (D) overexpressing Shark (hs-shark-10), (E) overexpressing kinase-dead Shark (hs-shark-10-K698R) and (F) overexpressing Src42A (hs-Src42A^22.3), together with cuticles of control, DC-defective, Ddok^PG155/Y embryos (left). +hs, heat shocked; -hs, no heat shock. Arrows indicate partial rescue of the dorsal open phenotype.