Fig. 7. Expression of tyrosine phosphorylated Shark at the periphery of epidermal cells is decreased in Ddok mutant embryos, during DC. (A,B) Embryo extract western blotted with (A) anti-pY927 and anti-phosphotyrosine (anti-P-Tyr-100) antibodies, and (B) anti-pY927, before (lane 1) and after (lane 2) phosphatase (CIAP) treatment of membrane, and with anti-Shark antiserum (lane 3). (C,D) Staining of wild-type and Ddok^PG155 mutant embryos with anti-pY927 Shark peptide antibody. Arrowheads indicate the LE.