Fig. 4. JIL-1 localization and kinase activity is not affected in Su(var)3-9 mutants. (A-D) Polytene squash preparations from male and female wild-type (wt) and Su(var)3-9¹/Su(var)3-9² (Su(var)3-9) third instar larvae labeled with JIL-1 antibody. Chromosome morphology and JIL-1 localization in Su(var)3-9¹/Su(var)3-9² preparations were similar to in wild-type preparations. (E) dmH3K9 levels were greatly reduced in Su(var)3-9¹/Su(var)3-9² mutants, whereas histone H3S10 phosphorylation was at wild-type levels. Immunoblots were performed on extracts from wild-type (wt) and Su(var)3-9¹/Su(var)3-9² (Su(var)3-9) third instar larvae. The immunoblots were labeled with anti-dmH3K9, anti-phospho-histone H3S10 (pH3S10) and anti-histone H3 antibodies. (F) dmH3K9 did not spread from the chromocenter to ectopic locations in polytene squashes from JIL-1^z2/JIL-1^z60 Su(var)3-9¹ (z2/z60, 3-9) larvae. The preparation was labeled with anti-dmH3K9 antibody (green) and Hoechst (DNA, blue). The X chromosome is indicated by X; the chromocenter by an arrow.