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Fig. 4. COGC-3 and COGC-1 immunoblotting. Western blot analysis of COGC-3 (A) and COGC-1 (B). Protein lysates prepared from wild-type, cogc-3 or cogc-1 worms were immunoblotted with anti-COGC-3 or anti-COGC-1. {alpha}-tubulin was used as a loading control. (C,D) Co-immunoprecipitation experiments. Protein lysate prepared from wild-type worms was immunoprecipitated with anti-COGC-3 or normal rabbit IgG (negative control) and then immunoblotted with anti-COGC-3 (C) or anti-COGC-1 (D).