Fig. 5. Expression of COGC-3. (A-C) Immunolocalization of COGC-3 in a cross-section of a wild-type L4 larva. The section was stained with anti-COGC-3 (red), fluorescein phalloidin (green) and DAPI (blue). (A) Merged image. (B) Anti-COGC-3 image. (C) Schematic drawing of the positions of tissues. Arrowhead indicates the perinuclear localization of COGC-3 in a muscle cell. The muscle cells contain actin filaments stained by fluorescein phalloidin apically and the nuclei basally. (D) Localization of COGC-3 in a cogc-3(k181) L4 larva. No COGC-3 signal was detected. Dorsal is towards the top. Scale bar: 12.5 µm. (E-J) Whole-mount immunohistochemistry with COGC-3 antibody. Antibody staining patterns (EG,I) and corresponding schematic drawings (F,H,J) of wild-type L4 larvae. (E,F) Pharynx, (G,H) vulva and (I,J) seam cell. Dorsal is towards the top. Anterior is leftwards. Scale bar: 25 µm.