Fig. 6. Immunocytochemistry of COGC-3. (A-C) Confocal images of a body wall muscle cell of an L4 larva transgenic for mig-23::GFP. (A) Anti-COGC-3, (B) anti-GFP and (C) a merged image. Some of the COGC-3 signals colocalized with MIG-23-GFP (arrowheads). (D-F) Confocal images of a body wall muscle cell of an L4 larva transgenic for YFP::TRAM. (D) Anti-COGC-3, (E) anti-GFP and (F) a merged image. YFP-TRAM and COGC-3 signals colocalized at the perinuclear region (small arrows). (G,H) Confocal images of MIG-23-GFP in body wall muscle cells in cogc-3(k181) L4 larvae. (G) Anti-COGC-3 and (H) anti-GFP. The number and intensity of MIG-23-GFP signals were reduced. A similar result was obtained in cogc-1(k179) (data not shown). (I-N) Confocal images of mRFP-SP12 (I,L) and MIG-23-GFP (J,M) and merged images (K,N) in body wall muscle cells in wild-type (I-K) and cogc-3(k181) (L-N) L4 larvae. MIG-23-GFP partially colocalizes with mRFP-SP12 in wild type, but not in cogc-3 mutants. The boundaries of the muscle cells are depicted by broken white lines. Scale bars: 25 µm. (O) Western blot of endogenous MIG-23. Protein lysates prepared from each strain were immunoblotted with anti-MIG-23 and anti--tubulin.