Fig. 2. Z-stack projections from live imaging time-lapse videos, showing changes in the axonal arbors of Tv neurons. (A) Enlarged still of the most anterior axon arbor (T1) at 6.5 hours apf, showing filopodia extending from the neurohemal organ. (B) Z-stack time-lapse projection of the T2 (bottom) and T3 (top) neurohemal organs (arrows) from 21 to 29 hours apf, every 60 minutes. Pruning can be seen clearly as T2 decreases in size from 21 to 29 hours apf. Filopodia from the growth zone under each neurohemal organ fasciculate (arrowheads). The last panel is an enlarged still of the T3 neurohemal organ at 23.5 hours apf, showing the large number of filopodia. (C) Z-stack time-lapse projection of a pruned T3 axon arbor from 25 to 28 hours apf, showing the dramatic outgrowth that occurs between 25 and 28 hours apf.