Development -- Shirato et al. 133 (2): 343 Figure IG2

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 2. Experimental protocol. (A) Immature oocyte. (B) The immature oocyte is bisected manually with a fine glass needle into a GV-containing and a non-nucleate fragment. (C) The GV-containing fragment is enucleated with a micropipette. This fragment is used as a centrosome donor. (D) Actinase-treated immature oocyte that is treated with 1-methyladenine (1-MeAde) to induce maturation. (E) Mature egg bearing both PB1 and PB2. (F) Conjugate of a non-nucleate fragment and a mature egg. Scale bar: 50 µm.