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Fig. 3. Immunofluorescent staining with an anti-{gamma}-tubulin antibody of a non-nucleate fragment. (A) Whole immature oocyte. Two spots (arrowheads) stained by the anti-{gamma}-tubulin antibody are located between the GV and the animal pole. (B) Enucleated fragment. Two spots (arrowheads) are stained by the anti-{gamma}-tubulin antibody. Scale bar: 25 µm.