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Fig. 4. Development of a conjugate at 22°C. The time (minutes) after activation is given in the upper right corner of each image. (A) The first cleavage. (B) The furrow regresses, and the conjugate remains as a single cell. (C) The second cleavage. Multiple furrows appear. (D) Four blastomeres form at the second cleavage. (E) The third cleavage forms eight blastomeres. Scale bar: 50 µm.