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Fig. 5. Nuclear events and the formation of mitotic asters in a Pattern 1 conjugate. The time (in minutes) after activation is given in the upper right corner of each image. Black arrows indicate bipolar spindles. White arrowheads indicate nuclei. (A) Pronucleus (PN) formation. (B) The first mitotic cycle. A bipolar spindle develops. (C) Two nuclei form. (D) The second mitotic cycle. Two bipolar spindles develop. (E) Four nuclei form. (F) The third mitotic cycle, forming four bipolar spindles. (G) Eight nuclei form. (H) The fourth mitotic cycle, forming eight bipolar spindles. (I) Sixteen nuclei form. (A,C,E,G,I) Differential interference-contrast microscopy; (B,D,F,H) polarization microscopy.