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Fig. 6. Nuclear events and the formation of mitotic asters in a Pattern 2 conjugate. The time (in minutes) after activation is given in the upper right corner of each image. Black arrows indicate monopolar or bipolar spindles; white arrowheads indicate nuclei; black arrowheads indicate single asters. (A) Pronucleus (PN) formation. (B) The first mitosis. One monopolar spindle and one aster develop [see Tamura and Nemoto (Tamura and Nemoto, 2001) for clearer pictures of monopolar spindles]. (C) One nucleus forms. (D) The second mitotic cycle. One bipolar spindle and two asters develop. (E) Two nuclei form. (F) The third mitotic cycle, forming two bipolar spindles and four asters. (G) Four nuclei formed. (a,c,e,g) Differential interference-contrast microscopy; (b,d,f) polarization microscopy.