Fig. 6. Molecular analysis of the optic vesicle and infundibulum in Raldh2^-/- embryos. (A,B) Whole-mount immunodetection of PAX6 protein in 10- to 11-somite stage (main panels) and 20- to 22-somite stage (insets) embryos. Profile views. Brackets indicate PAX6 expression in the dorsal telencephalon and diencephalon. (C-D') PAX6 immunohistochemistry on frontal sections through the optic vesicle (C,D) and telencephalon (C',D') of wild-type and Raldh2^-/- embryos (13-14 somites). (E-L) Whole-mount in situ hybridization of Six3 (E,F), Pax2 (G,H), Hesx1 (I,J) and Bmp4 (K,L) in 12- to 14-somite stage embryos. Genotypes are indicated above. Profile views. Insets in E,F show frontal views of Six6-hybridized embryos (brackets indicate infundibulum). Other insets show details of the optic vesicle of E9.5 embryos (G,H) or ventral views of the same embryos (I-L). ov, optic vesicle; te, telencephalon.