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Fig. 7. Molecular analysis of Fgf gene expression and signaling in Raldh2-/- embryos. (A-D) Whole-mount Fgf8 in situ hybridization on 13- to 14-somite stage (A,B) and E9.5 (C,D) embryos. Main panels: frontal views of the ANR. Insets show profile views (A,B) or transverse sections (C,D) of the same embryos. (E,F) Whole-mount p-ERK1/2 immunodetection (main panels, E9.5) and Mkp3 in situ hybridization (insets, 12- to 14-somite stage). Profile views. (G,H) Whole-mount Spry1 in situ hybridization on 12- to 14-somite stage embryos (main panels, profile views; insets, frontal views). Genotypes are indicated above. anr, anterior neural ridge; se, surface ectoderm.