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Figure 3


Fig. 3. Analysis of the ovarian {gamma}TuRC. (A) Expression analysis of {gamma}TuRC components and Swa in ovarian extracts of the indicated genotypes. (B) Anti-{gamma}Tub37C-immunoprecipitation from ovarian extracts of the indicated genotypes. (C-E) Wild-type (C), Grip75175 (D) and Grip128326 (E) homozygous mutant ovarian extracts were fractionated by 5-40% sucrose density gradient centrifugation. The first lane in each panel corresponds to the input, whereas subsequent lanes contain fractions obtained from top to bottom of the gradient.