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Figure 2


Fig. 2. Sema3d knockdown reduces migratory NCC markers without affecting EMT. (A,B) Uncaged FITC-dextran (green) and TOPRO3 labeling of nuclei (blue) in the hindbrain at 17 hpf in CONMO (A) and 3DMO (B) injected embryos. (C-E) Double in situ hybridization for crestin (green) and dlx2 (red); expression is reduced in 3DMO (D) and 3DI4E5MO (E) injected embryos compared with CONMO (C). (F) Quantification of the area of labeling of crestin and dlx2 in the r4 migratory stream, indicated by white outline in C. *P<10-6, t-test. (A-E) Dorsal views, anterior is leftwards. oto, otocyst. Scale bars: 40 µm.