Fig. 5. The tlr1 mutant phenotype can be rescued by expressing wild-type Tlr1 in various tissues. (A-F) Confocal micrographs of NMJs on ventral muscles 12, 13, 6 and 7 stained with CD8-GFP-Sh in undissected third instar larvae. Arrows indicate NMJs on muscles 12, 13, 6 and 7. (A) Wild type. (B) tlr1^D427/tlr1^K788 mutant larva carrying Heat shock-Gal4 and UAS-tlr1 transgenes. When no heat shock is applied, the neuromuscular pattern is disrupted. Muscles 12 and 13 are innervated at wrong positions, and the cleft between muscles 6 and 7 is only marginally innervated (arrows). (C) Application of a heat shock during mid-embryogenesis completely rescues the innervation errors on all muscles. (D) Ventral muscle field of a tlr1^D427/tlr1^K788 mutant larva expressing Tlr1 in all somatic muscles using G14-Gal4. The innervation pattern appears wild-type. (E) Expression of Tlr1 in all postmitotic neurons using Elav-Gal4 similarly rescues the innervation errors in tlr1^D427/tlr1^K788 mutants. (F) The neuromuscular pattern is not restored in tlr1^D427/tlr1^K788 mutants expressing Tlr1 only in cholinergic neurons using Cha-Gal4. NMJs on muscles 12 and 13 are mislocalized (arrows). Asterisks indicate NMJs located on muscles that are outside the intended focal plane.