Fig. 8. Shroom2 expression induces the apical accumulation of -tubulin in epithelial cells. (A-c') Both Shroom2 and Shroom3 expression in naïve blastomeres was found to trigger dramatic -tubulin accumulation at the apical cell surface of epithelial cells. (B-c') -Tubulin accumulation overlapped entirely with regions of pigment accumulation and had the same intensity in both Shroom2 and Shroom3-expressing embryos (1 ng injected for either mRNA). (D-f') Shroom2 expression (red) in naïve blastomeres did not co-localize with accumulated -tubulin (green), however, but localized at the cell surface basal to -tubulin.